Molecular Characterization of Human Immunodeficiency Virus (HIV)-1 and -2 in Individuals from Guinea-Bissau with Single or Dual Infections: Predominance of a Distinct HIV-1 Subtype A/G Recombinant in West Africa
Identifieur interne : 001199 ( Main/Exploration ); précédent : 001198; suivant : 001200Molecular Characterization of Human Immunodeficiency Virus (HIV)-1 and -2 in Individuals from Guinea-Bissau with Single or Dual Infections: Predominance of a Distinct HIV-1 Subtype A/G Recombinant in West Africa
Auteurs : Sören Andersson [Suède] ; Hans Norrgren [Guinée-Bissau] ; Francisco Dias [Guinée-Bissau] ; Gunnel Biberfeld [Suède] ; Jan Albert [Suède]Source :
- Virology [ 0042-6822 ] ; 1999.
Descripteurs français
- Wicri :
- topic : Base de données.
English descriptors
- KwdEn :
- Academic press, Aids surveillance, Alamo, Alamos database, Andersson, Biberfeld, Biophysics group, Bootstrap, Bootstrap support, Burkina faso, Cape verde, Carr, Cell samples, Central africa, Central african republic, Database, Database sequences, Distinct subtype, Dual infection, Dual infections, Dually, East africa, Epidemic spread, Gene sequences, Genetic analysis, Genetic data environment, Genetic subtypes, High concordance, Highest prevalence, Human immunodeficiency virus, Human immunodeficiency virus type, Ibng, Ibng cluster, Immune defic, Immunodeficiency, Infection, Infectious disease control, Ivory coast, Janssens, Leitner, Molecular characteristics, Molecular characterization, Molecular epidemiology, National hospital, Natural history, Neighbouring countries, Norrgren, Other parts, Other subtype, Other west african countries, Other west african countries benin, Outpatient, Peripheral blood, Phylogenetic, Phylogenetic analyses, Phylogenetic analysis, Phylogenetic tree, Polymerase chain reaction, Primer, Prospective cohort study, Recombinant, Reference sequences, Retrovirus, Search tools, Sequence clusters, Sequencing, Sequencing procedure, Sierra leone, Single infection, Single infections, Subtype, Subtype distribution, Subtype recombinants, Subtypes, Swedish institute, Theoretical biology, Tuberculosis hospital, Unlabeled sequences, Vaccine development, Variant, Virus variants, Weekly epidemiol, West africa, World health organization.
- Teeft :
- Academic press, Aids surveillance, Alamo, Alamos database, Andersson, Biberfeld, Biophysics group, Bootstrap, Bootstrap support, Burkina faso, Cape verde, Carr, Cell samples, Central africa, Central african republic, Database, Database sequences, Distinct subtype, Dual infection, Dual infections, Dually, East africa, Epidemic spread, Gene sequences, Genetic analysis, Genetic data environment, Genetic subtypes, High concordance, Highest prevalence, Human immunodeficiency virus, Human immunodeficiency virus type, Ibng, Ibng cluster, Immune defic, Immunodeficiency, Infection, Infectious disease control, Ivory coast, Janssens, Leitner, Molecular characteristics, Molecular characterization, Molecular epidemiology, National hospital, Natural history, Neighbouring countries, Norrgren, Other parts, Other subtype, Other west african countries, Other west african countries benin, Outpatient, Peripheral blood, Phylogenetic, Phylogenetic analyses, Phylogenetic analysis, Phylogenetic tree, Polymerase chain reaction, Primer, Prospective cohort study, Recombinant, Reference sequences, Retrovirus, Search tools, Sequence clusters, Sequencing, Sequencing procedure, Sierra leone, Single infection, Single infections, Subtype, Subtype distribution, Subtype recombinants, Subtypes, Swedish institute, Theoretical biology, Tuberculosis hospital, Unlabeled sequences, Vaccine development, Variant, Virus variants, Weekly epidemiol, West africa, World health organization.
Abstract
Guinea-Bissau in West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but recently the HIV-1 prevalence increased rapidly with the subsequent appearance of HIV-1 and HIV-2 dual infections. Information about the genetic subtypes of HIV in the region is limited. Therefore, we characterized the env V3 region of HIV-1 and HIV-2 variants through direct DNA sequencing of peripheral blood mononuclear cell samples from 18 individuals with HIV-1 only and 9 individuals with dual infection. Phylogenetic analyses of these new sequences and database sequences from other West African countries showed that all HIV-1 and HIV-2 sequences from singly as well as dually infected individuals, except one, clustered among HIV-1 subtype A and HIV-2 subtype A, respectively. Importantly, a majority of the HIV-1 sequences from Guinea-Bissau and neighbouring countries were closely related with the isolates IbNG, DJ263, and DJ264, which share a common subtype A/G recombination pattern. Analysis of pol gene sequences from selected HIV-1 variants showed that “IbNG-like” viruses in Guinea-Bissau are also recombinant, indicating that the HIV-1 epidemic in Guinea-Bissau and neighbouring countries is dominated by an epidemic spread of a distinct subtype A/G recombinant, which is strikingly similar to the epidemic spread of a subtype A/E recombinant in Southeast Asia. Furthermore, the HIV-1 and HIV-2 variants carried by individuals with dual infection were intermixed with variants from singly infected individuals, indicating that variants involved in dual and single infections have common epidemiological histories.
Url:
DOI: 10.1006/viro.1999.9867
Affiliations:
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samples</term><term>Central africa</term><term>Central african republic</term><term>Database</term><term>Database sequences</term><term>Distinct subtype</term><term>Dual infection</term><term>Dual infections</term><term>Dually</term><term>East africa</term><term>Epidemic spread</term><term>Gene sequences</term><term>Genetic analysis</term><term>Genetic data environment</term><term>Genetic subtypes</term><term>High concordance</term><term>Highest prevalence</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Ibng</term><term>Ibng cluster</term><term>Immune defic</term><term>Immunodeficiency</term><term>Infection</term><term>Infectious disease control</term><term>Ivory coast</term><term>Janssens</term><term>Leitner</term><term>Molecular characteristics</term><term>Molecular characterization</term><term>Molecular epidemiology</term><term>National hospital</term><term>Natural history</term><term>Neighbouring countries</term><term>Norrgren</term><term>Other parts</term><term>Other subtype</term><term>Other west african countries</term><term>Other west african countries benin</term><term>Outpatient</term><term>Peripheral blood</term><term>Phylogenetic</term><term>Phylogenetic analyses</term><term>Phylogenetic analysis</term><term>Phylogenetic tree</term><term>Polymerase chain reaction</term><term>Primer</term><term>Prospective cohort study</term><term>Recombinant</term><term>Reference sequences</term><term>Retrovirus</term><term>Search tools</term><term>Sequence clusters</term><term>Sequencing</term><term>Sequencing procedure</term><term>Sierra leone</term><term>Single infection</term><term>Single infections</term><term>Subtype</term><term>Subtype distribution</term><term>Subtype recombinants</term><term>Subtypes</term><term>Swedish institute</term><term>Theoretical biology</term><term>Tuberculosis hospital</term><term>Unlabeled sequences</term><term>Vaccine development</term><term>Variant</term><term>Virus variants</term><term>Weekly epidemiol</term><term>West africa</term><term>World health organization</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Base de données</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Guinea-Bissau in West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but recently the HIV-1 prevalence increased rapidly with the subsequent appearance of HIV-1 and HIV-2 dual infections. Information about the genetic subtypes of HIV in the region is limited. Therefore, we characterized the env V3 region of HIV-1 and HIV-2 variants through direct DNA sequencing of peripheral blood mononuclear cell samples from 18 individuals with HIV-1 only and 9 individuals with dual infection. Phylogenetic analyses of these new sequences and database sequences from other West African countries showed that all HIV-1 and HIV-2 sequences from singly as well as dually infected individuals, except one, clustered among HIV-1 subtype A and HIV-2 subtype A, respectively. Importantly, a majority of the HIV-1 sequences from Guinea-Bissau and neighbouring countries were closely related with the isolates IbNG, DJ263, and DJ264, which share a common subtype A/G recombination pattern. Analysis of pol gene sequences from selected HIV-1 variants showed that “IbNG-like” viruses in Guinea-Bissau are also recombinant, indicating that the HIV-1 epidemic in Guinea-Bissau and neighbouring countries is dominated by an epidemic spread of a distinct subtype A/G recombinant, which is strikingly similar to the epidemic spread of a subtype A/E recombinant in Southeast Asia. Furthermore, the HIV-1 and HIV-2 variants carried by individuals with dual infection were intermixed with variants from singly infected individuals, indicating that variants involved in dual and single infections have common epidemiological histories.</div></front></TEI><affiliations><list><country><li>Guinée-Bissau</li><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Andersson, Soren" sort="Andersson, Soren" uniqKey="Andersson S" first="Sören" last="Andersson">Sören Andersson</name></region><name sortKey="Albert, Jan" sort="Albert, Jan" uniqKey="Albert J" first="Jan" last="Albert">Jan Albert</name><name sortKey="Biberfeld, Gunnel" sort="Biberfeld, Gunnel" uniqKey="Biberfeld G" first="Gunnel" last="Biberfeld">Gunnel Biberfeld</name></country><country name="Guinée-Bissau"><noRegion><name sortKey="Norrgren, Hans" sort="Norrgren, Hans" uniqKey="Norrgren H" first="Hans" last="Norrgren">Hans Norrgren</name></noRegion><name sortKey="Dias, Francisco" sort="Dias, Francisco" uniqKey="Dias F" first="Francisco" last="Dias">Francisco Dias</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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